Prenatal diagnosis of β-thalassaemia in Mediterranean populations by dot blot analysis with DNA amplification and allele specific oligonucleotide probes

In this study, we describe a simple strategy to detect β-thalassaemia mutations in prospective parents and to make prenatal diagnosis in pregnancies at risk in the Mediterranean population. Screening of prospective parents is carried out by dot blot analysis on enzymatically amplified DNA with a set of oligonucleotide probes complementary to the most common mutations in this population. Prenatal diagnosis is accomplished by the same procedure on enzymatically amplified amniocyte or trophoblast DNA. The main advantages of this procedure are the simplicity, sensitivity (0.05 μg of DNA), and rapidity (12–24 h). Further simplification is obtained by amplification of the DNA from crude amniotic cell lysate. The very low amount of fetal material necessary for this analysis eliminates the need to culture amniotic fluid cells and may decrease the fetal loss rate associated with trophoblast sampling. The number of specific DNA sequences obtained by the amplification procedure allowed us to use non-radioactive labelled oligonucleotide probes, which have several advantages compared to radioactive probes.     

Effect of sorbed and desorbed Zn(Ⅱ) on the growth of a green alga (Chlorella pyrenoidosa)

Toxic effect of Zn(Ⅱ)on a green alga(ChloreUa pyrenoidosa)in the presence of sepiolite and kaolinite was investigated.The Zn-free clays were found to have a negative impact on the growth of C.pyrenoidosa in comparison with control samples(without adding any clay or Zn(Ⅱ)).When Zn(Ⅱ)was added,the algae in the presence of clays could be better survived than the control samples,which was actually caused by a decrease in Zn(Ⅱ)concentration in the solution owing to the adsorption of Zn(Ⅱ)on the clays.When the solution system was diluted,the growth of algae could be further inhibited as compared to that in a system which had the same initial Zn(Ⅱ)concentration as in the diluted system.This in fact resulted from desorption of Zn(Ⅱ)from the zinc-contaminated clays,although the effect varied according to the different desorption capabilities of sepiolite and kaolinite.Therefore the adsorption and desorption processes of Zn(Ⅱ)played an important part in its toxicity,and adsorption and desorption of pollutants on soils/sediments should be well considered in natural eco-environmental systems before their risk of toxicity to aquatic organisms was assessed objectively.     

贵阳市区大气降水中有机酸的研究

利用ICS-90常规离子色谱结合RFC-30型淋洗液在线发生器,对贵阳市区大气降水中的小分子有机酸进行了系统研究。测得贵阳市降水中的有机酸主要以甲酸[质量分数为(0.2-4.5)×10-6]、乙酸[质量分数为(0.6-5.3)×10-6]、草酸[质量分数为(0.1-4.9)×10-6]为主,其次是乳酸和丙酮酸,及少量丙酸和甲烷磺酸;有机酸对降水自由酸平均贡献为23.2%,占总阴离子的1.4%;通过比较贵阳市区有机酸前后二十年的变化,得出现阶段可能至少有近1/2乙酸和3/4甲酸来源于人类的活动,这说明有机酸人为源是其来源的重要组成部分。     

全氟辛烷磺酸盐在天然水体沉积物中的吸附-解吸行为

通过平衡振荡试验,探讨了沉积物的理化性质(总有机碳含量、粒度、阳离子交换容量、比表面积)、离子强度和pH值对全氟辛烷磺酸盐(PFOS)吸附-解吸行为的影响.结果表明,在中性环境中PFOS在水和沉积物中有机质之间的分配作用是影响其吸附行为的重要机制,分配系数Kd与沉积物中总有机碳含量呈显著正相关(r=0.96,p＜0.01,n=15).随着离子强度的增加,盯OS在沉积物中的吸附量明显增大,解吸滞后现象更加明显.pH的影响在酸性和碱性条件下(pH 4～8.5)呈现出不同的特点,在酸性条件下随pH值增加,PFOS在沉积物中的吸附量减少;在pH接近中性时达到最小值;在碱性条件下随pH增加,吸附量增加.     

煤矿轴流式局部通风机的降噪试验研究

煤矿局部通风机（简称“局扇”）的噪声是煤矿掘进中的主要噪声源，分析了局扇噪声的产生机理，提出了减少局扇的动叶叶顶与机壳间隙，在局扇入口加阻性消声器和在机壳涂上一层阻尼材料等方法来控制局扇的噪声方法，方法在JBT52－2型局扇的试验研究表明，实施上海降噪措施，局扇的噪声有大幅度降低，可使局扇的噪声达到工矿企业噪声卫生标准要求。     

大型构件防腐喷砂(涂)车间通风除尘设计

考虑到大型构件喷砂（涂）车间铝粉尘污染的特点以及构件进出空间的限制，比较了各种类型的除尘器，选择了最合理的通风除尘方案，进行了通风除尘系统的设计。该设计方案实施后，除尘效果显著。     

移动床生物膜反应器充氧能力的试验研究

对移动床生物膜反应器的充氧能力进行了试验研究，结果表明：反应器充氧能力在添加填料比无填料时大；在悬浮载体能够均匀流化的填充率范围内，反应器充氧能力随着填充率的增大而增大；当填充率大于可以均匀流化的最大填充率时，反应器的充氧能力略有下降。     

The Relation Between Sandstorms and Strong Winds in Xinjiang,China

With observational data spanning 1961–1999 from 90 meteorological stations in Xinjiang, China, the spatial and temporal characteristics of sandstorms and strong winds, and the contribution of strong winds to the occurrence of sandstormsare analyzed. Moreover, the dominant wind direction and minimumwind speeds during sandstorm periods are discussed. The research shows that although possessing similar climatic trends, sandstorms and strong winds in Xinjiang have opposite geographical distributions, i.e. places with more sandstorms show fewer strong winds. The contribution of strongwinds to sandstorms in northern Xinjiang is larger than that insouthern Xinjiang. The dominant wind directions clearly indicatethe paths of the weather systems that introduced the sandstorms.The minimum wind speeds in the sandstorms were over 10 ms^-1in northern and eastern Xinjiang and in the Turpan and Yanqi Basins of southern Xinjiang. In Tarim Basin of southern Xinjiang,however, the minimum wind speed was about 6–8 ms^-1, and even 6 ms^-1 at its southern edge.     

纺织废水臭味产生的原因和机理探讨

针对高硫酸盐、高色度纺织废水处理站恶臭气体所造成的环境污染,及对职工和居民的健康造成损害等一系列问题,进行了实验研究,分析了高硫酸盐、高色度纺织废水恶臭气体形成的原因和机理.     

Fenton法氧化降解聚乙烯醇的机制

为深入了解Fenton法氧化降解聚乙烯醇（PVA）的机制，通过考察反应过程中COD、BOD5、总有机碳及pH等随反应时间的变化，研究了聚乙烯醇Fenton氧化降解过程的特点，结果表明，当PVA被Fenton试剂氧化时，可生成大量CO2，同时生成酸性中间产物。进一步分析认为，当Fenton试剂中H2O2不足景时PVA氧化的中间产物以醛为主，而当H2O2足量时醛会被继续氧化生成有机酸，最终COD去除率达到80％。